Fiji manual (v6.3).pdf is publicly shared. CloudStor - CloudStor is powered by AARNet. If you are unsure how to go about it there is an example in the manual available here. Doing a simple percent area measuremnet follow a H DAB colour deconvolution is fine though. ¹This subreddit is not affiliated with the creators of ImageJ or FIJI, but is simply a place to share ideas, papers, resources, and expertise - especially as relate to questions & answers posted here. 11112 (Jumin Huang) May 28, 2020, 4:22am 1. Sign-up for one of the mailing lists: /Mailing_Lists It also hosts a forum for interacting with the developers.įIJI Is Just ImageJ - "a distribution of ImageJ (and ImageJ2) together with Java, Java3D and a lot of plugins organized into a coherent menu structure." is full of ImageJ development and analysis resources. Image analysis is interdisciplinary, so clearly explain field-specific terms or jargon. For example, immunofluorescent staining of a brain sections with NeuN/NSE (neuronal marker, Alexa 488, green color) and co-staining with calbindin D28K (Alexa 568, red color), I would like to. Clearly explain what you are trying to learn, not just the method used, to avoid the XY problem. Provide details: Be thorough in outlining the question(s) that you are trying to answer.People from the future may be stuck trying to answer the same question. Report spam or content that is hateful or off-topic.Upvote those who contribute to the discussion and provide freely of their time to assist you.Projects: Share a Link to your pet image analysis project.Research: Links to published (articles in scientific journals or in established repositories) that utilize ImageJ/FIJI for image analysis or are about image analysis.Discussions: Text posts, meant to ask about general issues relating to image analysis.Image analyst job posts are also welcome. Different methods are introduced in this tutorial.Timeline:00:00 Introducti. Tips: Text or Link posts to share useful how-to tricks and discoveries on using ImageJ/FIJI. Learn how to use FIJI (ImageJ) to create surface plots and surface render images.Questions which have been Solved will be marked as such. This could include algorithms, microscopy and scientific imaging, plug-ins, methods, and specific features of the software.
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